engleski

Improved proliferation of human osteosarcoma cells on oxygen plasma treated polystyrene

Polystyrene (PS) foils were exposed to oxygen plasma in order to improve the ability of human osteosarcoma cells (HOS) proliferation. Plasma was created in an electrodeless radio-frequency (RF) discharge in pure oxygen. Plasma treatment time of 30 s allowed for saturation of the surface with oxygen rich functional groups. Incubation of proteins from cell culture media was studied versus incubation time for untreated and plasma treated PS by X-ray photoelectron spectroscopy (XPS). Then HOS cells were cultivated according to a standard procedure, applied on the PS substrates and incubated. The proliferation was studied qualitatively by optical microscopy, scanning electron microscopy (SEM) and quantitatively by MTT assay. All techniques revealed significant differences in HOS cells proliferation on untreated and oxygen plasma treated PS. The differences were most pronounced after 24 h of incubation when the cells have already stared to proliferate on plasma treated PS, while they were still in quiescence on untreated PS. The confluency was achieved till the 6th day on plasma treated PS, while on untreated PS such confluency has never been achieved. The cell viability assay revealed that HOS cells grew better on plasma treated PS for various incubation times. Similar trend was observed regarding the adhesion of proteins. They adhered better on plasma treated PS causing better environment for further cell proliferation. The results clearly show that oxygen plasma treatment allows for much better proliferation of HOS cells on plasma treated PS as compared to the untreated PS substrates.

HOS cell proliferation; oxygen plasma; polystyrene; protein adsorption; surface modification

14th Joint Vacuum Conference/12th European Vacuum Conference/11th Annual Meeting of the Deutsche Vakuum Gesellschaft/ 19th Croatian-Slovenian Vacuum Meeting : proceedings ; skup je održan od 04.-08.06.2012.g., Dubrovnik, Hrvatska ; Nikola Radić, Slobodan Milošević i Branko Pivac (ur.).