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Pregled bibliografske jedinice broj: 663353

Časopis

Autori: Jørgensen, Peter; Milković, Lidija; Žarković, Neven; Waeg, Georg; Rattan, Suresh, I.S.
Naslov: Lipid peroxidation-derived 4-hydroxynonenal-modified proteins accumulate in human facial skin fibroblasts during ageing in vitro
Izvornik: Biogerontology (Dordrecht) (1389-5729) 15 (2013), 1; 105-110
Vrsta rada: članak
Ključne riječi: ageing; intervention; senescence; lipid peroxidation; molecular damage
Sažetak:
The reactive aldehyde, 4-hydroxynonenal (HNE), is recognized as a product of lipid peroxidation, which binds to macromolecules, in particular proteins. HNE-modified proteins (HNE-MP) have been shown to accumulate during ageing, generally by using polyclonal antibodies, which increase the possibility of detecting false positives. Therefore, we have used a genuine monoclonal antibody specific for HNE-His adducts of proteins/peptides, which were revealed by immunoblotting method for whole-cell HNE-MP measurements in serially passaged human facial skin fibroblasts undergoing ageing in vitro. There was a significant increase in the levels of HNE-MP in serially passaged cells approaching a near senescent state at high passage level (P-61), as compared with low passage level (P-11) young and middle-aged (P-27) cells. However, if the cells were analyzed soon after re-initiation from the frozen samples with little further passaging, the amount of HNE-MP was low even in relatively high passage level (P-37) cells, which is an indication of selective elimination of cells with high molecular damage during the process of thawing and re-initiation in culture. This pilot study on normal human facial skin fibroblasts shows that HNE-MP detection by monoclonal antibody-based dot blot method can be used as a marker for age-related accumulation of lipid peroxidative molecular damage, and could be useful for testing and monitoring the effects of potential skin care products on ageing parameters.
Projekt / tema: 098-0982464-2519
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kemija
URL Internet adrese: http://link.springer.com/article/10.1007%2Fs10522-013-9482-z
Broj citata:
Altmetric:
DOI: 10.1007/s10522-013-9482-z
URL cjelovitog teksta:
Google Scholar: Lipid peroxidation-derived 4-hydroxynonenal-modified proteins accumulate in human facial skin fibroblasts during ageing in vitro
Upisao u CROSBI: Lidija Milković (Lidija.Milkovic@irb.hr), 11. Pro. 2013. u 10:26 sati



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