engleski

Measurement of HNE-protein adducts in plasma and serum by ELISA

The HNE-protein adduct ELISA is a method to detect HNE bound to proteins, which is considered as the most likely form of HNE occurrence in living systems. The aim of this work was to adapt the ELISA to a commercial antibody, as well as for non-commercial antibody, and to apply it in the analysis of human plasma/serum samples. After modification and validation of the protocol with both antibodies, samples of two groups were analyzed: apparently healthy obese (n=62) and non-obese controls (n=15). Although the detected absolute values of HNE-protein adducts were different, depending on the antibody used, both ELISA methods showed significantly higher values of HNE-protein adducts in the obese group.

HNE-protein adducts; ELISA; plasma; serum

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