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Pregled bibliografske jedinice broj: 676248

Časopis

Autori: Kim, H.J.; ...; Galešić Ljubanović, Danica; Edelstein, C.L.
Naslov: NLRP3 inflammasome knockout mice are protected against ischemic but not cisplatin-induced acute kidney injury
( NLRP3 inflammasome knockout mice are protected against ischemic but not cisplatin-induced acute kidney injury )
Izvornik: The Journal of pharmacology and experimental therapeutics (0022-3565) 346 (2013), 3; 465-472
Vrsta rada: članak
Ključne riječi: Ischemic acute kidney injury; Cisplatin-induced acute kidney injury; NLRP3 inflammasome knockout mice
( Ischemic acute kidney injury; Cisplatin-induced acute kidney injury; NLRP3 inflammasome knockout mice )
Sažetak:
We have demonstrated that caspase-1 is a mediator of both cisplatin-induced acute kidney injury (AKI) and ischemic AKI. As caspase-1 is activated in the inflammasome, we investigated the inflammasome in cisplatin-induced and ischemic AKI. Mice were injected with cisplatin or subjected to bilateral renal pedicle clamping. Immunoblot analysis of whole kidney after cisplatin-induced AKI revealed: 1) an increase in apoptosis-associated Speck-like protein containing a caspase recruitment domain (ASC), the major protein that complexes with nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing proteins (NLRP) 1 or 3 to form the inflammasome ; 2) an increase in caspase- 1 activity, caspase-5, and NLRP1, components of the NLRP1 inflammasome ; and 3) a trend toward increased NLRP3. To determine whether the NLRP3 inflammasome plays an injurious role in cisplatin- induced AKI, we studied NLRP knockout (NLRP3(-/-)) mice. In cisplatin-induced AKI, the blood urea nitrogen, serum creatinine, acute tubular necrosis score, and tubular apoptosis score were not significantly decreased in NALP3(-/-) mice compared with wild-type mice. We have previously demonstrated the injurious role of caspase-1 in ischemic AKI. NLRP3, but not ASC or NLRP1, is increased in ischemic AKI. NLRP3(-/-) mice with ischemic AKI had significantly lower blood urea nitrogen, serum creatinine, and acute tubular necrosis and apoptosis scores than the wild-type controls. The difference in protection against cisplatin-induced AKI compared with ischemic AKI in NLRP3(-/-) mice was not explained by the differences in proinflammatory cytokines interleukin (IL)-1β, IL-6, chemokine (C-X-C motif) ligand 1, or tumor necrosis factor α. NLRP3 inflammasome is a mediator of ischemic AKI but not cisplatin-induced AKI, and further investigation of the NLRP1 inflammasome in cisplatin-induced AKI should prove interesting.
Projekt / tema: 198-0000000-3355
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kliničke medicinske znanosti
URL Internet adrese: http://jpet.aspetjournals.org/content/346/3/465
Broj citata:
Altmetric:
DOI: 10.1124/jpet.113.205732
URL cjelovitog teksta:
Google Scholar: NLRP3 inflammasome knockout mice are protected against ischemic but not cisplatin-induced acute kidney injury
Upisao u CROSBI: Danica Ljubanović (danica.ljubanovic@zg.t-com.hr), 8. Sij. 2014. u 18:38 sati



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