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 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 696105

Časopis

Autori: Simunić, Juraj; Petrov, Dražen; Bouceba, Tahar; Kamech, Nédia; Benincasa, Monica; Juretić, Davor
Naslov: Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA
Izvornik: Biochimica et biophysica acta. Biomembranes (0005-2736) 1838 (2014), 5; 1430-1438
Vrsta rada: članak
Ključne riječi: antimicrobial peptide; EST database; Trichoplax adhaerens; selectivity index; surface plasmon resonance; peptide-membrane interaction
Sažetak:
A method based on the use of signal peptide sequences from antimicrobial peptide (AMP) precursors was used to mine a placozoa expressed sequence tag database and identified a potential antimicrobial peptide from Trichoplax adhaerens. This peptide, with predicted sequence FFGRLKSVWSAVKHGWKAAKSR is the first AMP from a placozoan species, and was named trichoplaxin. It was chemically synthesized and its structural properties, biological activities and membrane selectivity were investigated. It adopts an α- helical structure in contact with membrane-like environments and is active against both Gram- negative and Gram-positive bacterial species (including MRSA), as well as yeasts from the Candida genus. The cytotoxic activity, as assessed by the haemolytic activity against rat erythrocytes, U937 cell permeabilization to propidium iodide and MCF7 cell mitochondrial activity, is significantly lower than the antimicrobial activity. In tests with membrane models, trichoplaxin shows high affinity for anionic prokaryote-like membranes with good fit in kinetic studies. Conversely, there is a low affinity for neutral eukaryote-like membranes and absence of a dose dependent response. With high selectivity for bacterial cells and no homologous sequence in the UniProt, trichoplaxin is a new potential lead compound for development of broad- spectrum antibacterial drugs.
Projekt / tema: 177-1770495-0476
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
SCI-EXP, SSCI i/ili A&HCI
Current Contents Connect (CCC)
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Scopus
Kategorija: Znanstveni
Znanstvena područja:
Fizika,Kemija,Biologija
URL Internet adrese: http://www.sciencedirect.com/science/article/pii/S0005273614000443
Broj citata:
Altmetric:
DOI: 10.1016/j.bbamem.2014.02.003
URL cjelovitog teksta:
Google Scholar: Trichoplaxin — A new membrane-active antimicrobial peptide from placozoan cDNA
Upisao u CROSBI: Davor Juretić (juretic@pmfst.hr), 7. Svi. 2014. u 11:56 sati
Napomene:
Highlights • First report of antimicrobial peptide from placozoa EST database • Broad activity spectrum against G − and G + bacteria, including MRSA • Low toxicity and high selectivity for prokaryotic- like membranes.



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