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Pregled bibliografske jedinice broj: 702313

Zbornik radova

Autori: Mandić, Magda; Mihevc, Andrej; Zubin Ferri, Tea; Ćetković, Helena; Pleše, Bruna; Krajcar Bronić, Ines
Naslov: MOONMILK IN POSTOJNA CAVE, SLOVENIA
Izvornik: Karst and microorganisms / Mulec, Janez (ur.). - Postojna : Karst research institute, Research Centre of the Slovenian Academy of Science and Arts , 2014. 38-38 (ISBN: 978-961-254-705-9).
Skup: 22nd International Karstological School "Classical Karst"
Mjesto i datum: Postojna, Slovenija, 16 - 20.06. 2014.
Ključne riječi: moonmilk ; bacteria ; petrography ; SEM micrographs ; DNA sequencing
Sažetak:
A comprehensive multidisciplinary study of Postojna Cave, the largest known cave in Europe and most visited cave in Slovenia, related to modern speleothem formation conditions that included one year monitoring of environmental conditions in the Cave and stable isotope analyses resulted in finding locations and samples which are promising candidates for paleoclimate investigations (Mandić, 2013). One of such samples is moonmilk from location Zgornji Tartar. Monitored environmental parameters of location microclimate and requirements for carbonate deposition under isotopic equilibrium on this location are fulfilled. Mean air temperature at location Zgornji Tartar is 10.7 ± 0.9 ºC and mean water temperature is 10.9 ± 1.0 ºC. Mean values of conductivity, pH, and pCO2 are 313 ± 123 µS/cm, 7.8 ± 0.3, and 937 ± 325 ppmv, respectively. Mean values of magnesium, calcium and bicarbonate concentrations are 0.8 ± 0.2 mg/L, 76 ± 4 mg/L, and 215 ± 24 mg/L, respectively. Because this is the first time that moonmilk is described in Postojna Cave, additional investigations helped its better characterization. Petrographicaly, the moonmilk is composed of calcite needles approximately 100 µm long, and 10 µm wide. SEM micrographs of moonmilk show evidence of microbial biomediation or bioprecipitation Also used Fourier transform infrared (FTIR) analysis indicate presence of organic compounds. Since Postojna Cave is touristic cave the question arose whether the organic compounds found in moonmilk sample are results of native cave microorganisms or if they are allochthonous species. Therefore, bacterial isolate DNA sequences were analyzed using BLAST, which identified sequence homologies with previously identified isolates. Sequences showing greater than 97% identity across the amplified region were considered to belong to the same operational taxonomic unit (OTU). Most of the sequences (68.4 %) were affiliated with the phylum Actinobacteria (species Streptomycetes). Uncultured bacteria from environmental samples were presented with 11.6% followed by Proteobacteria (1%) showing the highest identity with Klebsiella sp. thus indicating that microorganisms in moonmilk at the studied location are native. Mandić M., 2013. Determination of equilibrium conditions of carbonate precipitation in Postojna Cave with possible application to paleoclimatology. Ph.D. Thesis, Zagreb, University of Zagreb, 205 p.
Vrsta sudjelovanja: Pozvano
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Fizika,Geologija,Biologija
Upisao u CROSBI: Magda Mandić (mmandic@phy.uniri.hr), 26. Lip. 2014. u 14:40 sati



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