Adsorption of Proteins and Cell Adhesion to Plasma Treated Polymer Substrates (CROSBI ID 209679)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Recek, Nina ; Mozetič, Miran ; Jaganjac, Morana ; Milković, Lidija ; Žarković, Neven ; Vesel, Alenka
engleski
Adsorption of Proteins and Cell Adhesion to Plasma Treated Polymer Substrates
Plasma treatment is often used to alter cell interaction with polymer surfaces used in biomedical application. The influence of surface hydrophilicity/hydrophobicity on human mammary epithelial cell (HMEC) proliferation and adhesion of protein albumin to plasma treated polystyrene (PS) was studied. The PS surface was made hydrophilic or hydrophobic by treatment either in O2 or CF4 plasma. The rate of protein adhesion was studied by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) after incubation of PS in albumin solution for different periods, while cell viability and morphology was studied by MTT assay and scanning electron microscope (SEM). XPS measurements have shown that the quantity of adsorbed protein was higher for both plasma treated samples than for the untreated one. No significant difference regarding protein adhesion on hydrophilic or hydrophobic plasma treated surface was found by XPS. Contrary, the results for cell proliferation showed much better proliferation on hydrophilic surface.
biocompatibility ; cell adhesion ; oxygen and fluorine plasma treatment ; polymer surface modification ; protein adhesion
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Podaci o izdanju
63 (13)
2014.
685-691
objavljeno
0091-4037
10.1080/00914037.2013.854243