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Pregled bibliografske jedinice broj: 733452

Zbornik radova

Autori: Jain, Swati; Galešić Ljubanović, Danica; Edelstein, Charles L.; Jani, Alkesh
Naslov: The Changing Phenotype of Tubular Cell Death during Delayed Graft Function
( The Changing Phenotype of Tubular Cell Death during Delayed Graft Function )
Izvornik: J Am Soc Nephrol
ISSN: 1046-6673
Skup: ASN Kidney Week 2014
Mjesto i datum: Philadelphia, SAD, 11-16.11.2014
Ključne riječi: Tubular Cell Death; Delayed Graft Function
( Tubular Cell Death; Delayed Graft Function )
Sažetak:
Background: Delayed graft function (DGF) independently predicts reduced 5 yr kidney transplant survival. Treatments of DGF are lacking. Cold ischemia (CI) is a significant risk factor for DGF but the mechanism by which CI leads to DGF is unknown. The effect of CI alone versus CI + warm reperfusion (WR) on renal tubular cell (RTEC) death are not well defined. The aim of this study was to determine the relative effects of CI vs CI+WR on donor kidneys in a kidney transplant model of DGF. We hypothesized that CI alone would produce a different injury phenotype to CI+WR. Methods: Male C57BL6 mice aged 12 weeks were subjected to mouse kidney transplant. Donor kidneys subjected to 3 hours CI at 4°C in UW solution, were either processed immediately or subjected to syngeneic mouse kidney transplant. Renal function was assessed by serum creatinine (SCr). RTEC apoptosis and necrosis were assessed by a nephropathologist in a blinded fashion. Results: CI alone significantly increased RTEC apoptosis but did not result in necrosis. In contrast, CI+WR after transplant caused RTEC apoptosis and necrosis. SCr was significantly increased in transplants subjected to CI vs. no CI. Since caspase-8 is known to trigger apoptosis and also serves as a negative regulator of programmed necrosis, we examined caspase-8 protein expression and activity. Caspase-8 activity and protein expression were significantly decreased in CI+WR vs CI alone.Conclusions: We have shown that CI alone results in RTEC apoptosis whereas CI+WR after mouse kidney transplant results in a different injury phenotype with the development of RTEC necrosis. A potential mechanism by which these differing phenotypes may occur is via decreased caspase-8 after CI+WR, thus removing negative regulation of programmed necrosis. These studies suggest that while inhibition of capsase- 8 may prevent RTEC apoptosis, a consequence might be increased RTEC necrosis.
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 198-0000000-3355
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Kliničke medicinske znanosti
URL Internet adrese: https://www.asn-online.org/education/kidneyweek/archives/
Upisao u CROSBI: Danica Ljubanović (danica.ljubanovic@zg.t-com.hr), 24. Stu. 2014. u 20:02 sati



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