crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 741015

Časopis

Autori: Žižková, P.; Viskupičová, J.; Blaškovič, Dušan; Štrosová, M.K.; Žarković, Neven; Horáková, L.
Naslov: Sarcoplasmic reticulum Ca2+-ATPase from rabbit skeletal muscle modified by peroxynitrite
Izvornik: Journal of enzyme inhibition and medicinal chemistry (1475-6366) 29 (2014), 4; 563-570
Vrsta rada: članak
Ključne riječi: 4-Hydroxynonenal; calcium pump; carbonyls; oxidative stress
Sažetak:
Effect of peroxynitrite on SERCA1 activity was studied in correlation with enzyme carbonylation. Kinetic parameters and location of peroxynitrite effect on SERCA1 were determined. Carbonyls were determined by immunoblotting. FITC, NCD-4 and Trp fluorescence were used to indicate changes in cytosolic and transmembrane regions of SERCA1. Peroxynitrite-concentration-dependent decrease of SERCA1 activity was associated with elevation of protein carbonyls. 4-HNE was not involved in carbonylation of SERCA1. Increased FITC fluorescence in the presence of peroxynitrite correlated with the decrease of the enzyme affinity to ATP. Peroxynitrite-induced SERCA1 carbonylation that was not accompanied with the formation of 4-HNE-SERCA1 adducts is indicative of direct oxidation of SERCA1. As assessed by FITC fluorescence and decreased affinity of the enzyme to ATP, peroxynitrite impairment was found to occur in the cytosolic ATP-binding region of SERCA1.
Projekt / tema: 108-0000000-0028, 098-0982464-2519
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
URL Internet adrese: http://www.tandfonline.com/doi/full/10.3109/14756366.2013.827676
http://dx.doi.org/10.3109/14756366.2013.827676
Broj citata:
Altmetric:
DOI: 10.3109/14756366.2013.827676
URL cjelovitog teksta:
Google Scholar: Sarcoplasmic reticulum Ca2+-ATPase from rabbit skeletal muscle modified by peroxynitrite
Upisao u CROSBI: Marina Štoser (mstroser@mef.hr), 15. Pro. 2014. u 10:00 sati



  Verzija za printanje   za tiskati


upomoc
foot_4