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 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 744045

Disertacija

Autor: Kurolt, Ivan-Christian
Naslov: The influence of Puumala virus on the micro RNA expression in human epithelial kidney cells
( The influence of Puumala virus on the micro RNA expression in human epithelial kidney cells )
Vrsta: doktorska disertacija
Fakultet: Prirodoslovno-matematički fakultet
Sveučilište: Sveučilište u Zagrebu
Mjesto: Zagreb
Datum: 21.07.
Godina: 2014
Stranica: 187
Mentor: Markotić, Alemka ; Paessler, Slobodan
Ključne riječi: Puumala virus; micro RNA; HVBS
( Puumala virus; micro RNA; HFRS )
Sažetak:
This study investigates the expression or secretion of selected microRNAs (miRNAs) in vitro in HEK293 cells infected with Puumala virus (PUUV) or transiently transfected with PUUV N protein, and ex vivo in urine samples of patients with hemorrhagic fever with renal syndrome (HFRS) infected with PUUV, using real-time PCR. Limited deregulation of miR-21 and miR-27a expression was detected in HEK293, while miRNA abundance in supernatants was aberrant. Contrary to our hypothesis, transient expression of PUUV N did not yield significant changes in miRNA expression. In urinary samples of HFRS patients and patients with acute pyelonephritis, miR-21 and miR-27a were more abundant, while let-7e could be HFRS specific. In mild patients with HFRS only miR-21 and let-7e were deregulated, whereas in severe patients miR-24, miR-27a, and miR-127 were also altered. Detected miRNAs could present HFRS biomarkers correlating significantly with urea and creatinine levels, which represent hallmarks of HFRS progression and severity.
Projekt / tema: 143-1430115-0103
Izvorni jezik: eng
Znanstvena područja:
Biologija,Temeljne medicinske znanosti,Kliničke medicinske znanosti
Upisao u CROSBI: Lidija Cvetko (lcvetko@bfm.hr), 22. Pro. 2014. u 16:07 sati



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