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Pregled bibliografske jedinice broj: 771610

Časopis

Autori: Stipić, Filip; Burić, Petra; Jakšić, Željko; Pletikapić, Galja; Dutour Sikirić, Maja; Zgrablić, Goran; Frkanec, Leo; Lyons, Daniel Mark
Naslov: Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin
( Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin )
Izvornik: Colloids and surfaces. B, Biointerfaces (0927-7765) 135 (2015); 481-489
Vrsta rada: članak
Ključne riječi: Biosensor; diagnostic; nanoparticle; lanthanide; immunoassay; point-of-care
( Biosensor; diagnostic; nanoparticle; lanthanide; immunoassay; point-of-care )
Sažetak:
With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore : nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37–3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.
Projekt / tema: HRZZ-02.05/17
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Fizika,Kemija,Biologija
URL Internet adrese: http://www.sciencedirect.com/science/article/pii/S0927776515301119
http://dx.doi.org/10.1016/j.colsurfb.2015.07.077
Broj citata:
Altmetric:
DOI: 10.1016/j.colsurfb.2015.07.077
URL cjelovitog teksta:
Google Scholar: Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin
Upisao u CROSBI: Daniel Mark Lyons (Daniel.Mark.Lyons@irb.hr), 29. Srp. 2015. u 11:07 sati



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