Hrvatska znanstvena bibliografija (CROSBI)



Pregled bibliografske jedinice broj: 771757

Zbornik radova

Autori: Crnjac, Josip; Merkaš, Siniša; Rožić Sara; Sukser, Viktorija; Špoljarić, Daniel; Popović, Maja; Anđelinović, Šimun; Primorac, Dragan; Mršić, Gordan
Naslov: ANALYSIS OF 12 X- CHROMOSOME STR LOCI WITH REDUCED PCR SAMPLE VOLUME
Izvornik: 9th ISABS, Book of abstracts
Skup: 9th ISABS Conference on Forensic, Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine
Mjesto i datum: Bol, Hrvatska, 22-26.06.2015.
Ključne riječi: Forensics; DNA analysis; Investigator Argus X-12; X chromosome; Genetics
Sažetak:
STR markers on the X chromosome have emerged as additional tools in forensics, in missing person cases and parent-child relationships. Qiagen's Investigator® Argus X-12 kit for X- chromosome STR's, which analyses 12 loci (DXS10103, DXS8378, DXS7132, DXS10134, DXS10074, DXS10101, DXS10135, DXS7423, DXS10146, DXS10079, HPRTB, DXS10148) and amelogenin is currently being used. Following manufactures analysis protocol, obtained results from female samples were exhibiting spikes and pull- ups in electrophoresis, which were not found in male samples. Since the standard procedure requires samples to be normalized to concentrations of 1ng/µL of DNA before conducting PCR amplification, results from both male and female samples should be coherent. Due to observed spikes and pull-ups in female samples we diluted female and male normalized samples to 0.5ng/µL of DNA before setting up PCR reaction. Obtained results with diluted samples showed no spikes and pull-ups in female DNA profiles, while allele drop- out was observed in male DNA profiles. Thus we recommend normalization of female samples s to 0.5ng/µL of DNA rather than 1ng/µL of DNA asproposed by manufacturer.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Biologija,Temeljne medicinske znanosti
Upisao u CROSBI: Josip Crnjac (josip.crnjac@unist.hr), 31. Srp. 2015. u 12:46 sati