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Pregled bibliografske jedinice broj: 807639

Časopis

Autori: Andrišić, Luka; Collinson, Emma J.; Tehlivets, Oksana; Perak, Eleonora; Žarković, Tomislav; Dawes, Ian W.; Žarković, Neven; Čipak Gasparović, Ana
Naslov: Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast
Izvornik: Molecular and cellular biochemistry (0300-8177) 399 (2014), 1/2; 27-37
Vrsta rada: članak
Ključne riječi: catalase; Desaturase; Fatty acid β-oxidation; PUFA; ROS
Sažetak:
Pathophysiology of polyunsaturated fatty acids (PUFAs) is associated with aberrant lipid and oxygen metabolism. In particular, under oxidative stress, PUFAs are prone to autocatalytic degradation via peroxidation, leading to formation of reactive aldehydes with numerous potentially harmful effects. However, the pathological and compensatory mechanisms induced by lipid peroxidation are very complex and not sufficiently understood. In our study, we have used yeast capable of endogenous PUFA synthesis in order to understand the effects triggered by PUFA accumulation on cellular physiology of a eukaryotic organism. The mechanisms induced by PUFA accumulation in S. cerevisiae expressing Hevea brasiliensis Δ12-fatty acid desaturase include down-regulation of components of electron transport chain in mitochondria as well as up-regulation of pentose-phosphate pathway and fatty acid β-oxidation at the transcriptional level. Interestingly, while no changes were observed at the transcriptional level, activities of two important enzymatic antioxidants, catalase and glutathione-S-transferase, were altered in response to PUFA accumulation. Increased intracellular glutathione levels further suggest an endogenous oxidative stress and activation of antioxidative defense mechanisms under conditions of PUFA accumulation. Finally, our data suggest that PUFA in cell membrane causes metabolic changes which in turn lead to adaptation to endogenous oxidative stress.
Projekt / tema: 108-0000000-0028, 098-0982464-2519
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Biologija,Temeljne medicinske znanosti
URL Internet adrese: http://www.springer.com/life+sciences/biochemistry+%26+biophysics/journal/11010?cm_mmc=sgw-_-ps-_-journal-_-11010
http://link.springer.com/article/10.1007%2Fs11010-014-2229-6
Broj citata:
Altmetric:
DOI: 10.1007/s11010-014-2229-6
URL cjelovitog teksta: http://www.springer.com/life+sciences/biochemistry+%26+biophysics/journal/11010?cm_mmc=sgw-_-ps-_-journal-_-11010
Google Scholar: Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast
Upisao u CROSBI: Ana Čipak Gašparović (Ana.Cipak.Gasparovic@irb.hr), 29. Ožu. 2016. u 15:02 sati



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