Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast (CROSBI ID 226408)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Andrišić, Luka ; Collinson, Emma J. ; Tehlivets, Oksana ; Perak, Eleonora ; Žarković, Tomislav ; Dawes, Ian W. ; Žarković, Neven ; Čipak Gasparović, Ana Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast // Molecular and cellular biochemistry, 399 (2015), 1/2; 27-37. doi: 10.1007/s11010-014-2229-6

Podaci o odgovornosti

Andrišić, Luka ; Collinson, Emma J. ; Tehlivets, Oksana ; Perak, Eleonora ; Žarković, Tomislav ; Dawes, Ian W. ; Žarković, Neven ; Čipak Gasparović, Ana

engleski

Transcriptional and antioxidative responses to endogenous polyunsaturated fatty acid accumulation in yeast

Pathophysiology of polyunsaturated fatty acids (PUFAs) is associated with aberrant lipid and oxygen metabolism. In particular, under oxidative stress, PUFAs are prone to autocatalytic degradation via peroxidation, leading to formation of reactive aldehydes with numerous potentially harmful effects. However, the pathological and compensatory mechanisms induced by lipid peroxidation are very complex and not sufficiently understood. In our study, we have used yeast capable of endogenous PUFA synthesis in order to understand the effects triggered by PUFA accumulation on cellular physiology of a eukaryotic organism. The mechanisms induced by PUFA accumulation in S. cerevisiae expressing Hevea brasiliensis Δ12-fatty acid desaturase include down-regulation of components of electron transport chain in mitochondria as well as up-regulation of pentose-phosphate pathway and fatty acid β-oxidation at the transcriptional level. Interestingly, while no changes were observed at the transcriptional level, activities of two important enzymatic antioxidants, catalase and glutathione-S-transferase, were altered in response to PUFA accumulation. Increased intracellular glutathione levels further suggest an endogenous oxidative stress and activation of antioxidative defense mechanisms under conditions of PUFA accumulation. Finally, our data suggest that PUFA in cell membrane causes metabolic changes which in turn lead to adaptation to endogenous oxidative stress.

catalase ; Desaturase ; Fatty acid β-oxidation ; PUFA ; ROS

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

399 (1/2)

2015.

27-37

objavljeno

0300-8177

1573-4919

10.1007/s11010-014-2229-6

Povezanost rada

Biologija, Temeljne medicinske znanosti

Poveznice
Indeksiranost