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Pregled bibliografske jedinice broj: 807823

Časopis

Autori: Łuczaj, Wojciech; Moniuszko, Anna; Jarocka-Karpowicz, Iwona; Pancewicz, Sławomir; Andrišić, Luka; Žarković, Neven; Skrzydlewska, Elżbieta
Naslov: Tick-borne encephalitis – lipid peroxidation and its consequences
Izvornik: Scandinavian journal of clinical & laboratory investigation (0036-5513) 76 (2016), 1; 1-9
Vrsta rada: članak
Ključne riječi: 4-hydroxynonenal ; CSF ; isoprostanes ; MDA ; phospholipase A2 ; plasma ; urine
Sažetak:
Background: The purpose of this study was to assess the processes of lipid peroxidation with prostaglandin derivatives and reactive aldehydes being its major indicators in cerebrospinal fluid (CSF), plasma and urine of patients with tick-borne encephalitis (TBE). Materials and methods: This study included 60 patients with TBE and 56 healthy subjects. Lipid peroxidation was estimated by the measurement of 4-hydroxynonenal (4-HNE), 4-hydroxyhexenal (4-HHE), malondialdehyde (MDA), acrolein, crotonaldehyde, and 4-oxononenal (4-ONE), determined by GC-MS, F2-isoprostanes and neuroprostanes (NPs) level determined by LC-MS. The level of 4-HNE-protein adducts was determined by ELISA. Phospholipase A2 (PLA2), platelet-activating factor acetylhydrolase (PAF-AH) and glutathione peroxidase (GSH-Px) activities and vitamin E level were determined spectrophotometrically and by HPLC, respectively. In parallel, the plasma levels of phospholipid acids such as arachidonic acid (AA), linoleic acid (LA) and docosahexaenoic acid (DHA) were monitored. Results: A significant decrease in AA, LA, DHA level and GSH-Px activity (by about 20, 69, 11 and 18%, respectively) was observed. The consequence of enhanced phospholipid peroxidation was almost 7 times higher plasma level of F2-isoprostanes and 3-fold increase in NPs level in CSF of TBE patients. Additionally a 3.5-fold increase in the CSF level of MDA, 5-fold increase in the plasma level of 4-HNE and urine level of 4-HHE in TBE patients was observed. Decreased plasma activity of PLA2 with an increase in the PAF-AH activity was observed. Conclusion: Lipid peroxidation occurring during TBE development indicates its relevance in pathophysiology of this disease. Moreover lipid peroxidation products might be useful for the diagnosis of TBE.
Projekt / tema: 098-0982464-2519
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Broj citata:
Altmetric:
DOI: 10.3109/00365513.2015.1084040
URL cjelovitog teksta:
Google Scholar: Tick-borne encephalitis – lipid peroxidation and its consequences
Upisao u CROSBI: Lidija Milković (Lidija.Milkovic@irb.hr), 30. Ožu. 2016. u 13:54 sati



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