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izvor podataka: crosbi

Tick-borne encephalitis – lipid peroxidation and its consequences (CROSBI ID 226451)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Łuczaj, Wojciech ; Moniuszko, Anna ; Jarocka-Karpowicz, Iwona ; Pancewicz, Sławomir ; Andrišić, Luka ; Žarković, Neven ; Skrzydlewska, Elżbieta Tick-borne encephalitis – lipid peroxidation and its consequences // Scandinavian journal of clinical & laboratory investigation, 76 (2016), 1; 1-9. doi: 10.3109/00365513.2015.1084040

Podaci o odgovornosti

Łuczaj, Wojciech ; Moniuszko, Anna ; Jarocka-Karpowicz, Iwona ; Pancewicz, Sławomir ; Andrišić, Luka ; Žarković, Neven ; Skrzydlewska, Elżbieta

engleski

Tick-borne encephalitis – lipid peroxidation and its consequences

Background: The purpose of this study was to assess the processes of lipid peroxidation with prostaglandin derivatives and reactive aldehydes being its major indicators in cerebrospinal fluid (CSF), plasma and urine of patients with tick-borne encephalitis (TBE). Materials and methods: This study included 60 patients with TBE and 56 healthy subjects. Lipid peroxidation was estimated by the measurement of 4-hydroxynonenal (4-HNE), 4-hydroxyhexenal (4-HHE), malondialdehyde (MDA), acrolein, crotonaldehyde, and 4-oxononenal (4-ONE), determined by GC-MS, F2-isoprostanes and neuroprostanes (NPs) level determined by LC-MS. The level of 4-HNE-protein adducts was determined by ELISA. Phospholipase A2 (PLA2), platelet-activating factor acetylhydrolase (PAF-AH) and glutathione peroxidase (GSH-Px) activities and vitamin E level were determined spectrophotometrically and by HPLC, respectively. In parallel, the plasma levels of phospholipid acids such as arachidonic acid (AA), linoleic acid (LA) and docosahexaenoic acid (DHA) were monitored. Results: A significant decrease in AA, LA, DHA level and GSH-Px activity (by about 20, 69, 11 and 18%, respectively) was observed. The consequence of enhanced phospholipid peroxidation was almost 7 times higher plasma level of F2-isoprostanes and 3-fold increase in NPs level in CSF of TBE patients. Additionally a 3.5-fold increase in the CSF level of MDA, 5-fold increase in the plasma level of 4-HNE and urine level of 4-HHE in TBE patients was observed. Decreased plasma activity of PLA2 with an increase in the PAF-AH activity was observed. Conclusion: Lipid peroxidation occurring during TBE development indicates its relevance in pathophysiology of this disease. Moreover lipid peroxidation products might be useful for the diagnosis of TBE.

4-hydroxynonenal ; CSF ; isoprostanes ; MDA ; phospholipase A2 ; plasma ; urine

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Podaci o izdanju

76 (1)

2016.

1-9

objavljeno

0036-5513

1502-7686

10.3109/00365513.2015.1084040

Povezanost rada

nije evidentirano

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