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Pregled bibliografske jedinice broj: 821724

Časopis

Autori: Jain, Swati; Keys, Daniel; Galešić Ljubanović, Danica; Edelstein, Charles L.; Jani, Alkesh
Naslov: Protection Against Cold Storage-Induced Renal Tubular Cell Apoptosis
( Protection Against Cold Storage-Induced Renal Tubular Cell Apoptosis )
Izvornik: Transplantation (0041-1337) 99 (2015), 11; 2311-2316
Vrsta rada: članak
Ključne riječi: Prolonged cold storage ; Renal Tubular Cell Apoptosis
( Prolonged cold storage ; Renal Tubular Cell Apoptosis )
Sažetak:
BACKGROUND: Prolonged cold storage (CS) of donor kidneys is associated with tubular cell apoptosis and caspase-3 activation. We have previously shown that pancaspase inhibition prevents CS-associated tubular apoptosis. Because of the nonspecific nature of pancaspase inhibitors, which block all caspases including proinflammatory caspase-1, the effect of specific caspase-3 inhibition during CS is unknown. X-linked inhibitor of apoptosis (XIAP) is the most potent naturally occurring specific inhibitor of caspase-3. We hypothesized that prolonged CS would decrease XIAP, whereas upregulation of XIAP with the novel compound UCF- 101 would protect against caspase-3 activation and tubular cell apoptosis. METHODS: LLC-PK1 tubular cells and whole kidneys from C57BL/6 mice were subjected to prolonged CS with or without UCF-101, and examined for XIAP, caspase-3, and tubular apoptosis. RESULTS: Tubular cells subjected to prolonged CS in vitro demonstrated significantly decreased XIAP and significantly increased apoptosis, caspase-3 protein and activity. UCF-101 treatment significantly increased XIAP, significantly decreased capsase-3 protein and activity, and protected against apoptosis. To determine the therapeutic significance, whole kidneys were subjected to prolonged CS with UCF-101. UCF-101 significantly increased XIAP in donor kidneys and protected against apoptosis. CONCLUSIONS: Prolonged CS of tubular cells in vitro and whole mouse kidneys ex vivo is associated with loss of XIAP and subsequent tubular cell apoptosis. UCF- 101 protects against the loss of XIAP during prolonged CS both in vitro and ex vivo, and is associated with significantly reduced tubular cell apoptosis. UCF-101 may represent an attractive approach to improve organ preservation.
Projekt / tema: 198-0000000-3355
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kliničke medicinske znanosti
Broj citata:
Altmetric:
DOI: 10.1097/TP.0000000000000774
URL cjelovitog teksta:
Google Scholar: Protection Against Cold Storage-Induced Renal Tubular Cell Apoptosis
Upisao u CROSBI: Danica Ljubanović (danica.ljubanovic@zg.t-com.hr), 15. Lip. 2016. u 19:16 sati



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