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Pregled bibliografske jedinice broj: 822923

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Autori: Šenjug, Petar; Krištić, Anteja; Bauer Šegvić, Anamarija; Bacalja, Jasna; Bulimbašić, Stela; Galešić Ljubanović, Danica
Naslov: Standardization of measurement and determination of normal glomerular basement membrane thickness at Department of Pathology and Cytology, Dubrava University Hospital, Zagreb
( Standardization of measurement and determination of normal glomerular basement membrane thickness at Department of Pathology and Cytology, Dubrava University Hospital, Zagreb )
Izvornik: Virchows Archiv
ISSN: 0945-6317
Skup: 27th European Congress of Pathology
Mjesto i datum: Beograd, Srbija, 05-09.09.2015
Ključne riječi: Glomerular basement membrane ; electron microscopy
( Glomerular basement membrane ; electron microscopy )
Sažetak:
Objective. Glomerular basement membrane (GBM) thickness depends on multiple factors and many authors emphasize need for referential span of normal GBM thickness for each laboratory. Methods. We have applied modification of the direct measurement/arithmetic mean method to measure GBM thickness on digital EM photographies (30 measurements for each biopsy) using iTEM software (Olympus Soft Imaging Solutions GmbH). Position of measurements in capillary loops was determined similarly as described by Haas (Arch Pathol Lab Med, 2009). Results. We obtained mean ±SD values for the normal GBM thickness of adult males and females of 340±36 nm and 301±44 nm. These values were based on average GBM thickness for each of 23 males and 22 females, aged 19 to 84 years, with minimal change disease, acute interstitial nephritis, normal renal parenchyma or acute tubular injury. Exclusion criteria were hematuria and diabetes mellitus. We defined normal ranges for each gender (268-412 nm for males, 213-389 for females), as being within 2 SD of these means. Conclusion. With this research we have standardized method for GBM thickness measurement and defined referential span of normal GBM thickness for our laboratory. These results are crucial for accurate diagnosis of disorders characterized with structural changes of GBM.
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Vrsta sudjelovanja: Predavanje
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: HRZZ-IP-2014-09-2151
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Kliničke medicinske znanosti
Upisao u CROSBI: Danica Ljubanović (danica.ljubanovic@zg.t-com.hr), 21. Lip. 2016. u 20:51 sati



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