crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 827035

Časopis

Autori: Žunec, Suzana; Kašuba, Vilena; Pavičić, Ivan; Marjanović, Ana Marija; Tariba, Blanka; Milić, Mirta; Kopjar, Nevenka; Pizent, Alica; Lucić Vrdoljak, Ana; Rozgaj, Ružica; Želježić, Davor
Naslov: Assessment of oxidative stress responses and the cytotoxic and genotoxic potential of the herbicide tembotrione in HepG2 cells
( Assessment of oxidative stress responses and the cytotoxic and genotoxic potential of the herbicide tembotrione in HepG2 cells )
Izvornik: Food and chemical toxicology (0278-6915) 94 (2016); 64-74
Vrsta rada: članak
Ključne riječi: DNA damage ; genome instability ; 4-Hydroxyphenyl-pyruvate-dioxygenase ; oxidative stress ; toxicity ; triketone herbicide
( DNA damage ; genome instability ; 4-Hydroxyphenyl-pyruvate-dioxygenase ; oxidative stress ; toxicity ; triketone herbicide )
Sažetak:
Tembotrione is a triketone herbicide, usually used for post-emergence weed control in corn. Currently, there is little or no published data on its genotoxicity to human cells either in vitro or in vivo. This study evaluated the impact of acute (4 and 24 h) exposure to low concentrations of tembotrione [corresponding to the acceptable daily intake (0.17 mg/mL), residential exposure level (0.002 mg/mL) and acceptable operator exposure level (0.0012 mg/mL)] on human hepatocellular carcinoma cell line HepG2, using biomarkers of oxidative stress, CCK-8 colorimetric assay for cell viability, alkaline comet assay, and cytokinesis-block micronucleus “cytome” assay. Tembotrione applied at concentrations likely to be encountered in occupational and residential exposures induced cytogenetic outcomes in non- target cells despite non-significant changes in the values of oxidative stress biomarkers. We assume that the observed effects were mainly the consequence of impaired metabolic pathways in HepG2 cells due to the inhibition of the enzyme 4-hydroxyphenyl-pyruvate-dioxygenase by tembotrione, which possibly caused a depletion of folate levels leading to excess formation of nuclear buds in the affected cells. Regardless of the fact that tembotrione was previously reported negative for mutations and chromosome aberrations in vitro, our findings call for more precaution in its use.
Projekt / tema: HRZZ-IP-2013-11-8366
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
URL Internet adrese: http://www.sciencedirect.com/science/article/pii/S0278691516301740
Broj citata:
Altmetric:
DOI: 10.1016/j.fct.2016.05.019
URL cjelovitog teksta:
Google Scholar: Assessment of oxidative stress responses and the cytotoxic and genotoxic potential of the herbicide tembotrione in HepG2 cells
Upisao u CROSBI: Davor Želježić (dzeljezi@imi.hr), 13. Srp. 2016. u 15:37 sati



  Verzija za printanje   za tiskati


upomoc
foot_4