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Pregled bibliografske jedinice broj: 83907

Zbornik radova

Autori: Čačev, Tamara; Spaventi, Radan; Pavelić, Krešimir; Kapitanović, Sanja
Naslov: Genetic changes of APC, beta-catenin and E-cadherin genes in colon cancer
Izvornik: Abstracts of the 18th UICC International Cancer Congress in Internetional Journal of CancerOslo : Wiley-Liss, Inc. , 2002. 319-320.
ISSN: 0020-7136
Skup: 18th UICC International Cancer Congress
Mjesto i datum: Oslo, Norveška, 30.06.-05.07.2002.
Ključne riječi: APC; beta-catenin; E-cadherin; colon cancer
Sažetak:
Background: Activation of APC/beta-catenin signalling pathway by mutation in either the APC or beta-catenin gene contributes to colorectal carcinogenesis. E-cadherin is involved in control and maintenance of normal intercellular adhesion and acts as a strong invasion supressor. We examined 60 cases of human sporadic colon cancer and corresponding normal tissue samples to evaluate the loss of heterozygosity (LOH) and presence of mutations at the APC, beta-catenin and E-cadherin gene loci. Methods: DNAs were used for PCR, RFLP, VNTR and LOH analysis. To analyze LOH at the APC gene loci we used three RFLP intragenic markers (exon 11 RsaI, exon 15 MspI, and exon 15 AspHI). The presence of the mutations in the 15H amplicon of the APC gene, and most frequent APC gene mutation in codon 1309 were analyzed as well. To analyze mutations in the beta-catenin gene we amplified exon 3 and the entire intronic sequences flanking it from genomic DNAs of all 60 tumors. For the LOH analysis of E-cadherin gene locus we used D16S752 polymorphic marker. Results: The informativity for all three APC intragenic markers was 53.3 % (32 of 60 assayed), and 25 % of tumors (8 of 32 informative) demonstrated LOH. We found two APC gene mutations in our tumor samples: a 5bp deletion in codon 1309, and an insetrion in the 15H amplicon of the APC gene. In 3.3 % of tumor samples (2 of 60 tested) the mutation of the beta-catenin gene was found. The informativity of D16S752 E-cadherin gene polymorphic marker was 75% (45 of 60 tested) and 28.8 % of tumors (13 of 45 informative) demonstrated LOH. Conclusion: Our study showed that activation of the APC/beta-catenin signalling and loss of E-cadherin function are important events in colorectal cancerogenesis.
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MEDLINE
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SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 00981104, 0098109, 0098108, 0098093
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti,Kliničke medicinske znanosti



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