Expression of polysialic acid in primary laryngeal squamous cell carcinoma (CROSBI ID 237218)
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Klobučar, Marko ; Visentin, Sarah ; Jakovčević, Antonija ; Bilić, Mario ; Kovač-Bilić, Lana ; Đanić, Davorin ; Pavelić, Krešimir ; Kraljević Pavelić, Sandra
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Expression of polysialic acid in primary laryngeal squamous cell carcinoma
AIMS: Expression of polySia is associated with metastatic dissemination and progression of various malignant diseases. In particular, it may contribute to tumorigenesis by a negative modulatory effect on cellular signaling cascades responsible for cellular migration, differentiation and proliferation. In this study, we investigated the expression of polySia in primary metastatic and non- metastatic laryngeal squamous cell carcinoma (LSCC) tumor tissues and its potential impact on the LSCC progression. MAIN METHODS: The expression of polySia in metastatic and non- metastatic primary laryngeal squamous cell carcinoma (LSCC) tumor biopsy specimens was investigated by immunohistochemistry, while the expression of polysialyltransferase IV (ST8SiaIV) (), fibroblast growth factor receptor 1 (FGFR1), extracellular signal regulated kinases 1 and 2 (Erk 1/2) and c-Raf was tested in metastatic and non-metastatic primary tumor tissues (including the corresponding non-tumor control tissues) by Western blot analysis. KEY FINDINGS: The expression of polySia was detected in LSCC biopsies specimens with generally stronger immunoreactivity in non-metastatic tumor LSCC sections and in histologically undifferentiated tumors. Also, increased polySia expression was observed in adjacent histologically unaltered laryngeal tumor-associated tissue of the metastatic sections. In addition, we provide an evidence of increased polysialyltransferase IV (ST8SiaIV) expression, involved in polySia synthesis in both metastatic and non-metastatic primary tumors which is accompanied by decreased levels of FGFR1, Erk 1/2 and c-Raf. SIGNIFICANCE: We present for the first time the evidence for the polySia expression in LSCC biopsies specimens which suggests its potential impact on initial steps of LSCC malignant transformation.
FGFR-MAPK/Erk1/2signaling ; Laryngeal squamous cell carcinoma ; Metastases ; Polysialic acid ; Polysialyltransferase
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