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Pregled bibliografske jedinice broj: 888600

Časopis

Autori: Tariba Lovaković, Blanka; Pizent, Alica; Kašuba, Vilena; Kopjar, Nevenka; Micek, Vedran; Mendaš, Gordana; Dvoršćak, Marija; Mikolić, Anja; Milić, Mirta; Žunec, Suzana; Lucić Vrdoljak, Ana; Želježić, Davor
Naslov: Effects of sub-chronic exposure to terbuthylazine on DNA damage, oxidative stress and parent compound/metabolite levels in adult male rats
( Effects of sub-chronic exposure to terbuthylazine on DNA damage, oxidative stress and parent compound/metabolite levels in adult male rats )
Izvornik: Food and chemical toxicology (0278-6915) 108 (2017), Part A; 93-103
Vrsta rada: članak
Ključne riječi: Chlorotriazine herbicide ; Antioxidant enzymes ; Genotoxicity ; HPLC-UV DAD analysis ; Lipid peroxidation ; Total antioxidant capacity
( Chlorotriazine herbicide ; Antioxidant enzymes ; Genotoxicity ; HPLC-UV DAD analysis ; Lipid peroxidation ; Total antioxidant capacity )
Sažetak:
Terbuthylazine is a selective pre- and post- emergency chloro-triazine herbicide used for a broad spectrum of weed control. We evaluated the potential of low doses of terbuthylazine to induce oxidative stress and cytogenetic damage in peripheral blood samples of adult male Wistar rats. Following 28-day repeated oral exposure at 0.004 mg/kg b.w./day, 0.4 mg/kg b.w./day and 2.29 mg/kg b.w./day, parameters of lipid peroxidation, total antioxidant capacity, and activities of antioxidant enzymes were measured in blood samples. Alkaline comet assay on leukocytes and erythrocyte micronucleus assay were used to measure DNA damage. In addition, the concentration of terbuthylazine and its metabolite in urine and plasma were determined using high performance liquid chromatography with UV diode-array detector (HPLC-UV-DAD). The fraction of terbuthylazine excreted in urine was negligible and was not found in plasma. Deethylterbuthylazine was only compound detected in plasma samples. Exposure to terbuthylazine did not induce significant lipid peroxidation products. The significant changes in antioxidant enzyme activities and the elevated total antioxidant capacity indicated that terbuthylazine at experimental conditions applied has potential to disturb oxidative/antioxidant balance. Results regarding the alkaline comet assay as well as micronucleated reticulocyte frequency indicated that treatment led to low-level DNA instability. Our results call for further research using other sensitive biomarkers of effect, along with different exposure scenarios.
Projekt / tema: HRZZ-IP-2013-11-8366
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Biologija,Javno zdravstvo i zdravstvena zaštita
URL Internet adrese: https://doi.org/10.1016/j.fct.2017.07.046
http://www.sciencedirect.com/science/article/pii/S0278691517304283?via%3Dihub
Broj citata:
Altmetric:
DOI: 10.1016/j.fct.2017.07.046
URL cjelovitog teksta:
Google Scholar: Effects of sub-chronic exposure to terbuthylazine on DNA damage, oxidative stress and parent compound/metabolite levels in adult male rats
Upisao u CROSBI: Alica Pizent (apizent@imi.hr), 16. Kol. 2017. u 09:48 sati



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