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Gas chromatographic-mass spectrometric analysis of urinary volatile organic metabolites: Optimization of the HS-SPME procedure and sample storage conditions (CROSBI ID 241683)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Živković Semren, Tanja ; Brčić Karačonji, Irena ; Safner, Toni ; Brajenović, Nataša ; Tariba Lovaković, Blanka ; Pizent, Alica Gas chromatographic-mass spectrometric analysis of urinary volatile organic metabolites: Optimization of the HS-SPME procedure and sample storage conditions // Talanta, 176 (2018), 537-543. doi: 10.1016/j.talanta.2017.08.064

Podaci o odgovornosti

Živković Semren, Tanja ; Brčić Karačonji, Irena ; Safner, Toni ; Brajenović, Nataša ; Tariba Lovaković, Blanka ; Pizent, Alica

engleski

Gas chromatographic-mass spectrometric analysis of urinary volatile organic metabolites: Optimization of the HS-SPME procedure and sample storage conditions

Non-targeted metabolomics research of human volatile urinary metabolome can be used to identify potential biomarkers associated with the changes in metabolism related to various health disorders. To ensure reliable analysis of urinary volatile organic metabolites (VOMs) by gas chromatography-mass spectrometry (GC- MS), parameters affecting the headspace-solid phase microextraction (HS-SPME) procedure have been evaluated and optimized. The influence of incubation and extraction temperatures and times, coating fibre material and salt addition on SPME efficiency was investigated by multivariate optimization methods using reduced factorial and Doehlert matrix designs. The results showed optimum values for temperature to be 60 °C, extraction time 50 min, and incubation time 35 min. The proposed conditions were applied to investigate urine samples’ stability regarding different storage conditions and freeze-thaw processes. The sum of peak areas of urine samples stored at 4 °C, −20 °C, and −80 °C up to six months showed a time dependent decrease over time although storage at −80 °C resulted in a slight non- significant reduction comparing to the fresh sample. However, due to the volatile nature of the analysed compounds, more than two cycles of freezing/thawing of the sample stored for six months at −80 °C should be avoided whenever possible.

Volatile organic metabolites ; Urine samples ; HS-SPME procedure ; Sample stability ; Metabolomic

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Podaci o izdanju

176

2018.

537-543

objavljeno

0039-9140

10.1016/j.talanta.2017.08.064

Povezanost rada

Kemija

Poveznice