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FT-IR spectroscopy as a method to detected cell surface components of Gluconobacter oxydans involved in the bacteria-OTA interaction

Fourier Transform Infrared Spectroscopy (FT-IR) is an analytical technique, which provides unique spectrum for each bacterial species due to the stretching and bending vibrations of functional groups present in cell proteins, nucleic acids, lipids, sugars, and lipopolysaccharides. The potential of Gluconobacter oxydans to bind ochratoxin A (OTA) on surface and changes occurring on the cell surface due to mycotoxin exposure were investigated using the FT-IR. OTA was determined using liquid chromatography tandem mass spectrometry (LC-MS/MS) and the results suggest that G. oxydans is able to bind or degrade OTA by 81%. The results revealed capacity of OTA exposed cells to bind a high amount of the toxins is mirrored in the alteration of the original IR patterns, the latter alteration arising due the presence of numerous functional groups within the toxin structure. Major changes seen in the 1, 800-1, 600 cm-1, 1, 600-1, 450 cm-1 and 1, 300-1, 000 cm-1 regions, mainly correspond to carbonyl, aromatic and ether groups, respectively. These groups are abundant in OTA and increase the existing, or provide new spectrum bands. Additionally, very strong signals in the organic halogen compounds region at 870 cm–1, probably corresponds to the C-C1 vibration taking place in OTA.

Gluconobacter oxydans, OTA, cell wall components, FT-IR

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