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Pregled bibliografske jedinice broj: 912243

Zbornik radova

Autori: Štimac, Adela; Tokić, Matea; Ljubetič, Ajasja; Vuletić, Tomislav; Frkanec, Leo; Šekutor, Marina; Frkanec, Ruža
Naslov: FCS testing of DNA interaction with cyclodextrine based supramolecular systems functionalized with adamantyl guanidine
Izvornik: Book of abstracts, 12th Christmas Biophysics Workshop / Jan Rozman, Primož Ziherl (ur.). - Ljubljana :
Skup: 12th Christmas Biophysics Workshop
Mjesto i datum: Golte, Slovenija, 11.-12.12.2017.
Ključne riječi: adamantyl guanidine, amphiphilic beta-cyclodextrine, supramolecular complexes, liposomes, host-guest interaction, fluorescence correlation spectroscopy
Sažetak:
The aim of the present study was preparation of cyclodextrine based supramolecular systems functionalized with adamantyl guanidine and probing of their interaction with DNA using fluorescence correlation spectroscopy (FCS). We prepared and characterized supramolecular systems based on phosphatidylcholine liposome and vesicles of amphiphilic β-cyclodextrine (CD) derivative functionalized by entrapment of a series of adamantyl guanidines AG 1-5. Efficiency of incorporation of adamantyl guanidines into prepared supramolecular systems was measured spectrophotometrically, and the size and surface charge were determined by dynamic light scattering method. It was shown that all examined compounds were successfully incorporated into prepared supramolecular systems except compound AG5. The highest encapsulation efficiency was demonstrated for compound AG1. The incorporation of adamantyl guanidines did not affect the size of prepared vesicles but the vesicle charge was significantly changed. FCS was applied to examine the interactions between functionalized vesicles and the Cy5- fluorescently labelled, double-stranded, 120 bp DNA (DNA120*). The obtained results have clearly demonstrated that the vesicles with entrapped AG 1-4 present guanidinium groups on the surface of vesicles, which leads to interaction with DNA120* via guanidine- phosphate interaction. Also, it was shown that vesicles modified with adamantyl guanylhydrazones (AG 1-2) bind DNA more efficiently, for almost an order of magnitude than vesicles modified with adamantyl aminoguanidines (AG 3-4). Preliminary results have shown that prepared functional supramolecular systems strongly bind DNA and as such could be used in gene delivery.
Vrsta sudjelovanja: Predavanje
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Domaća recenzija
Projekt / tema: HRZZ-IP-2013-11-7387
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Fizika,Kemija,Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Upisao u CROSBI: Adela Štimac (astimac@unizg.hr), 13. Pro. 2017. u 09:44 sati



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