engleski

Phenotypic and functional characteristics of human first trimester decidual macrophages and dendritic cells

Human early pregnancy decidua harbors immunocompetent CD45+ cells, such as natural killer cells (NK), macrophages, dendritic cells (DCs) and T cells. Macrophages are professional phagocytes which assist in initiating and facilitating cell-mediated immune responses against pathogens. Bone marrow-derived DCs are the most potent activators of naive T-lymphocytes responses. Since macrophages and dendritic cells in the decidua are located in the close position to the decidual lymphocytes (NK), it is reasonable to suppose that they could participate, directly or by paracrine mechanism, in the regulation of their cytolytic function. The aim of this study was to determine phenotype of decidual macrophages and dendritic cells present in the different culture conditions of human first trimester decidua, as well as their interaction with decidual NK cells. Studies were performed on freshly isolated or cultured decidual cells (adherent and non-adherent fraction). Phenotype of first trimester decidual macrophages and dendritic cells was analysed by flow cytometry and immunocytochemistry. HLA-DR depleted and HLA-DR non-depleted cells were used as possible modulators of perforin (P) expression and cytotoxicity of decidual lymphocytes (DL). We have shown previously that decidual adherent cells (DAC) are composed of a mixture of various cell populations: 20% of DAC are CD45+ cells and 80% of DAC are CD45- and at the same time vimentin+, cytokeratin-, 61537 ; ; -actin+ and epidermal growth factor receptor (EGFR) +, indicating that they are decidual stromal cells. Almost all peripheral blood adherent cells (PBAC) (cca 90%) expressed monocyte/macrophage markers but only 10-20% of DAC. We have identified CD83+ DCs in adherent, as well as non-adherent cell fraction of cultured decidual cells. It has been proven that CD83 is a suitable and selective cell-surface marker for mature DCs. Mature DCs express HLA-DR, as well as CD86 surface molecule. We have shown that the percentage of P+ cells significantly decreases in the suspension of fresh non-stimulated decidual lymphocytes (DL), but this was prevented in the presence of DAC. Contrary to DAC, HLA-DR depleted DAC can not prevent the loss of perforin in DL. Cytolytic activity of DL against NK sensitive cell line - K562 was decreased after addition of DAC supernatant. Decidual dendritic cells (CD83+) and macrophages are suitable candidates that mediate the balance of maternal defensive immune responses to foreign antigens. These cells could also participate in induction of some aspects of tolerance to the conceptus in the human decidua through interaction with decidual NK cells.

human decidua; macrophages; dendritic cells; CD83; CD86

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