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Pregled bibliografske jedinice broj: 931636

Časopis

Autori: Kopjar, Nevenka; Žunec, Suzana; Mendaš Starčević, Gordana; Micek, Vedran; Kašuba, Vilena; Mikolić, Anja; Tariba Lovaković, Blanka; Milić, Mirta; Pavičić, Ivan; Marjanović Čermak, Ana Marija; Pizent, Alica; Lucić Vrdoljak, Ana; Želježić, Davor
Naslov: Evaluation of chlorpyrifos toxicity through a 28-day study: Cholinesterase activity, oxidative stress responses, parent compound/metabolite levels, and primary DNA damage in blood and brain tissue of adult male Wistar rats
( Evaluation of chlorpyrifos toxicity through a 28-day study: Cholinesterase activity, oxidative stress responses, parent compound/metabolite levels, and primary DNA damage in blood and brain tissue of adult male Wistar rats )
Izvornik: Chemico-biological interactions (0009-2797) 279 (2018); 51-63
Vrsta rada: članak
Ključne riječi: acetylcholinesterase ; genotoxicity ; HPLC-UV DAD analysis ; organophosphate pesticide ; oxidative stress
( acetylcholinesterase ; genotoxicity ; HPLC-UV DAD analysis ; organophosphate pesticide ; oxidative stress )
Sažetak:
In this 28 day-study, we evaluated the effects of the insecticide chlorpyrifos orally administered to Wistar rats at doses 0.160, 0.015, and 0.010 mg/kg b. w./day. Following treatment, total cholinesterase activity and activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were measured. Oxidative stress responses were evaluated using a battery of endpoints to establish lipid peroxidation, changes in total antioxidant capacity, level of reactive oxygen species (ROS), glutathione (GSH) level and activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase. Using HPLC-UV DAD analysis, levels of the parent compound and its main metabolite 3, 5, 6-trichloro-2-pyridinol in plasma and brain tissue were measured. The genotoxic effect was estimated using alkaline comet assay in leukocytes and brain tissue. The exposure did not result in significant effects on total cholinesterase, AChE and BChE activity in plasma and brain tissue. Lipid peroxidation slightly increased both in plasma and brain tissue. Total antioxidant capacity, ROS and GSH levels were marginally influenced by the exposure. Treatment led to significant increases of GSH-Px activity in blood, SOD activity in erythrocytes and a slight increase of catalase activity in plasma. HPLC-UV DAD analysis revealed the presence of both the parent compound and its main metabolite in the plasma of all of the experimental animals and brain tissue of the animals treated at the two higher doses. All of the tested doses of chlorpyrifos were slightly genotoxic, both to leukocytes and brain tissue. Our results call for further research using other sensitive biomarkers of effect, along with different exposure scenarios.
Projekt / tema: HRZZ-IP-2013-11-8366
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kemija,Biologija,Javno zdravstvo i zdravstvena zaštita
Puni text rada: 931636.chlorpyrifos.pdf (tekst priložen 21. Ožu. 2018. u 09:29 sati)
URL Internet adrese: https://www.ncbi.nlm.nih.gov/pubmed/29108776
https://www.sciencedirect.com/science/article/pii/S0009279717309985?via%3Dihub
Broj citata:
Altmetric:
DOI: 10.1016/j.cbi.2017.10.029
URL cjelovitog teksta:
Google Scholar: Evaluation of chlorpyrifos toxicity through a 28-day study: Cholinesterase activity, oxidative stress responses, parent compound/metabolite levels, and primary DNA damage in blood and brain tissue of adult male Wistar rats
Upisao u CROSBI: Mirta Milić (mmilic@imi.hr), 21. Ožu. 2018. u 09:29 sati



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