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Immediate early response of the marine sponge Suberites domuncula to heat stress: Reduction of trehalose and glutathione concentrations and glutathione S-transferase activity

The marine sponge Suberites domuncula was used to identify early markers for thermal stress. Cubes from sponges have been kept for 30 min at 31oC (10oC higher then the ambient temperature). After this treatment the sponge cubes were kept again at 21oC. To demonstrate that animals reacted to the elevated temperature, the expression of heat shock protein (HSP) was determined. Using an antibody raised against HSP70, it was found by Western blotting that the animals specifically express a 45 kDa polypeptide after heat treatment. It was shown that even after 10 min of heat treatment the steady-state concentration of trehalose drops by 40% from abase level of 13 nmol/mg protein. The activity of trehalose-degrading enzyme, trehalase, remained unchanged. Additional early biomarkers for thermal stress include the enzyme activity of glutathione S-transferase (GST) and the concentration of glutathione (GSH). After 5-min the activity of GST decreased by 40%. Similarly, the concentration of GSH dropped by 50% after 15 to 20 min exposure. The original levels of biomarkers, trehalose, GSH and GST, were reached again after a recovery period of about 180 min. By contrast, the steady-state concentration of polyphosphates did not change during heat treatment. These data show that in S.domuncula the concentrations of trehalose, GSH, and the activity of GST, are biomarkers for immediate early response towards heat stress.

sponge; Suberites domuncula; heat shock; trehalose; glutathione; S-transferase

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