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Pregled bibliografske jedinice broj: 973902

Zbornik radova

Autori: Rinčić, Nikolina; Sabol, Maja; Trnski, Diana; Musani, Vesna; Ozretić, Petar; Levanat, Sonja
Naslov: Responsiveness to GANT-61 inhibitor of GLI1, GLI2 and GLI3 knock-out ovarian cancer cell lines
( Responsiveness to GANT-61 inhibitor of GLI1, GLI2 and GLI3 knock-out ovarian cancer cell lines )
Izvornik: Libri Oncologici : Croatian Journal of Oncology, Vol. 46 No. Supplement 1, 2018. / Vrdoljak, Danko Velimir (ur.). - Zagreb : Klinika za tumore, Klinički bolnički centar Sestre milosrdnice , 2018. 81-81.
ISSN: 0300-8142
Skup: "HDIR-5: Translating Science to Medicine - Targets and Therapeutics" - Fifth Meeting of the Croatian Association for Cancer Research with International Participation
Mjesto i datum: Zagreb, Hrvatska, 8.-10.11.2018.
Ključne riječi: Hedgehog signaling, ovarian cancer, CRISPR-Cas9, GANT
( Hedgehog signaling, ovarian cancer, CRISPR-Cas9, GANT )
Sažetak:
Hedgehog signaling pathway is a developmental signaling pathway which is dormant in most adult differentiated tissues, but aberrantly activated in various tumors. In ovarian tumors it can be activated in a canonical way, by the SHH ligand, or the non-canonical way, by upregulation of the GLI transcription factors. Usually, only GLI1 expression was associated with tumor progression, but GLI2 and GLI3 also play a role by modifying the activity of GLI1 and transcription of their common transcriptional targets. In ovarian cancer, GLI3 protein is expressed in the full-length activator form, and not the shortened repressor form which is the predominant form for GLI3 protein. To study the roles of the three GLI proteins in detail, we used the CRISPR/Cas9 guided gene editing to generate knock-out lines for each of the three GLI proteins. The knock- out was confirmed by Western blot. The modified cell lines showed differences in cell morphology compared to the original SKOV3 cell line immediately after expansion from the single cell, as well as different response to GANT-61 inhibition on cell cycle, apoptosis and colony forming capabilities of the cell lines. This demonstrates that all three GLI proteins are important for survival of ovarian cancer, and not only GLI1 as was considered previously.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Domaća recenzija
Projekt / tema: HRZZ-IP-2016-06-1268
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Biologija,Temeljne medicinske znanosti
URL Internet adrese: https://hrcak.srce.hr/file/305661
Upisao u CROSBI: Maja Sabol (Maja.Sabol@irb.hr), 13. Pro. 2018. u 10:46 sati



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