Responsiveness to GANT-61 inhibitor of GLI1, GLI2 and GLI3 knock-out ovarian cancer cell lines (CROSBI ID 670652)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Rinčić, Nikolina ; Sabol, Maja ; Trnski, Diana ; Musani, Vesna ; Ozretić, Petar ; Levanat, Sonja
engleski
Responsiveness to GANT-61 inhibitor of GLI1, GLI2 and GLI3 knock-out ovarian cancer cell lines
Hedgehog signaling pathway is a developmental signaling pathway which is dormant in most adult differentiated tissues, but aberrantly activated in various tumors. In ovarian tumors it can be activated in a canonical way, by the SHH ligand, or the non-canonical way, by upregulation of the GLI transcription factors. Usually, only GLI1 expression was associated with tumor progression, but GLI2 and GLI3 also play a role by modifying the activity of GLI1 and transcription of their common transcriptional targets. In ovarian cancer, GLI3 protein is expressed in the full-length activator form, and not the shortened repressor form which is the predominant form for GLI3 protein. To study the roles of the three GLI proteins in detail, we used the CRISPR/Cas9 guided gene editing to generate knock-out lines for each of the three GLI proteins. The knock- out was confirmed by Western blot. The modified cell lines showed differences in cell morphology compared to the original SKOV3 cell line immediately after expansion from the single cell, as well as different response to GANT-61 inhibition on cell cycle, apoptosis and colony forming capabilities of the cell lines. This demonstrates that all three GLI proteins are important for survival of ovarian cancer, and not only GLI1 as was considered previously.
Hedgehog signaling ; ovarian cancer ; CRISPR-Cas9 ; GANT
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Podaci o prilogu
81-81.
2018.
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objavljeno
Podaci o matičnoj publikaciji
Libri oncologici : Croatian journal of oncology
Ozretić, Petar ; Levanat, Sonja
Zagreb: Klinički bolnički centar Sestre milosrdnice
0300-8142
2584-3826
Podaci o skupu
5th Meeting of the Croatian Association for Cancer Research with International Participation: Translating Science to Medicine "Targets and Therapeutics" (HDIR-5)
poster
08.11.2018-10.11.2018
Zagreb, Hrvatska