engleski

The effect of silver nanoparticles on oxidative stress in tobacco seedlings (Nicotiana tabacum)

Nanomaterials are widely used in everyday life, and silver nanoparticles (AgNPs) are often applied. They provide better utilization of antibacterial and antifungal effects of silver. Numerous studies conducted to determine their toxic effect on ecosystems, and therefore on plants, are in the focus of interest. Tobacco is a model plant often used in abiotic stress studies. In this research the effects of AgNPs on tobacco seedlings were analyzed. Seedlings were also exposed to the same concentrations of ionic silver (AgNO3). For the evaluation of oxidative stress the content of malondialdehyde (MDA) and protein carbonyls as well as the activity of antioxidative enzymes pyrogallol (PPX), ascorbate peroxidase (APX) and catalase (CAT) were analyzed spectrophotometrically and in‐gel. Compared to control, all AgNO3 treatments led to a decrease of MDA, and the same trend was obser ved after exposure to the higher AgNP concentrations. The increase of carbonyls was recorded in seedlings treated with AgNPs, while exposure to AgNO3 showed no change. Generally, PPX and CAT activity was reduced after the AgNP and AgNO3 treatments, although the decrease of PPX activity was more expressed in AgNO3 treatments. Increased activity of APX was determined in all treatments. The results revealed that oxidative stress participates in the toxicity mechanism of AgNPs and suggests that involvement of plants in the comprehensive research of nanoparticles toxicity is convenient and necessary.

tobacco; nanosilver; oxidative stres

nije evidentirano