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Pregled bibliografske jedinice broj: 426201

Časopis

Autori: Režek Jambrak, Anet; Mason, Timothy J.; Lelas, Vesna; Krešić, Greta
Naslov: Ultrasonic effect on physicochemical and functional properties of α -lactalbumin
Izvornik: LWT- Lebensmittel-Wissenschaft und Technologie- Food Science and Technology (0023-6438) 43 (2010); 254-262
Vrsta rada: članak
Ključne riječi: ultrasound treatment; α -lactalbumin; physicochemical properties; functional properties; particle size; molecular weight
Sažetak:
Ultrasound is the sound whose frequency is too high for humans to hear which is within the frequency range of 20 Hz-20 kHz, and the frequency of ultrasound is above 20 kHz. The aim of this study was to observe the effect of ultrasound and sonication on α -lactalbumin (α -LA) with a view to improving its physicochemical and functional properties. In this work both low-intensity ultrasound (500 kHz bath) and the high-intensity ultrasound (20 kHz probe and 40 kHz bath) were used. Ten per cent wt (g g-1 dry matter) protein model suspensions of α -lactalbumin (α -LA) were treated with ultrasound probe (20 kHz for 15 and 30 minutes) and ultrasound baths (40 kHz and 500 kHz for 15 and 30 minutes). Changes in pH values, electrical conductivity, solubility measurements, foaming properties, as well as rheological and freezing-thawing properties have been examined. The protein fractions of α -lactalbumin were analyzed before and after ultrasound treatment by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The result showed that pH did not change significantly upon ultrasound however conductivities increased significantly after 20 kHz sonication. Electrical conductivity decreased significantly for ultrasound treatments in baths at 40 kHz and 500 kHz for all samples. Solubility increased significantly for all samples at 20 kHz. Foam capacities and foam stabilities were improved after ultrasound treatments for both 20 kHz and 40 kHz treatments. Foaming properties were not improved for protein model suspensions for 500 kHz treatments. The molecular weight of the protein decreased significantly after ultrasound treatments both using a 20 kHz probe and 40 kHz bath. The flow behavior of α -lactalbumin was observed to be shear- thickening after all treatments. Apparent viscosity data calculated with power law equation (R2 = 0.983– 0.999) have not been changed significantly after all treatments. A remarkable decrease of initial freezing point was obtained after 20 kHz treatments.
Projekt / tema: 058-0580696-2533
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Prehrambena tehnologija
Broj citata:
Altmetric:
DOI: 10.1016/j.lwt.2009.09.001
URL cjelovitog teksta:
Google Scholar: Ultrasonic effect on physicochemical and functional properties of α -lactalbumin
Upisao u CROSBI: arezek@pbf.hr (arezek@pbf.hr), 8. Ruj. 2009. u 14:06 sati



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