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Pregled bibliografske jedinice broj: 830694

Zbornik radova

Autori: Oros, Damir; Andjelković, Uroš; Kraljević Pavelić, Sandra; Josić, Djuro; Čeprnja, Marina; Melvan, Ena; Žučko, Jurica; Starčević, Antonio
Naslov: Identification of pathogens from urine samples by MALDI-TOF/TOF
Izvornik: EMBO Workshop, 10th European Summer School / Küster B., Lemeer S., Marcus K., Urlaub H. (ur.). -
Skup: "Advanced Proteomics"
Mjesto i datum: Brixen/Bressanone, Italija, 31.7. - 6.8.2016.
Ključne riječi: Urine culture; MALDI-TOF/TOF; pathogen identification; proteomics
Sažetak:
Fast and reliable microbial pathogen identification procedure in human urine samples is a crucial diagnostics step for all clinical microbiology laboratories, especially due to a growing antibiotic resistance concern. Currently used identification methods in a clinical set-up are often lengthy and may lead to prescription of unnecessary or inadequate antibiotic therapy. Therefore, we developed a new and robust MALDI-TOF/TOF mass spectrometry- based method coupled to bioinformatics analysis for identification of microorganisms and quantification of peptides from urine samples, readily transferable into a clinical environment. Bioinformatics analysis relied on database peptide matching and species identification by use of newly, in-house developed software based on natural language processing. In the presented study we evaluated this newly developed protocol for analysis of 40 human urine samples previously screened in the clinical microbiology laboratory as pathogen-positive and with concentrations higher than 10000 c.f.u. 1/ml. As a reference for bacterial diversity, seven randomly selected urine samples were determined using 16S rRNA gene sequencing. Our results show that MALDI-TOF/TOF MS proved useful for determination of bacteria in urine samples and that obtained result highly depend on the sample preparation method, starting material properties and number of MS spectra used for the MSMS analysis.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 02.05/60 „Klinička proteomika mikroorganizama”
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Biotehnologija
Upisao u CROSBI: Damir Oros (doros@pbf.hr), 31. Kol. 2016. u 00:47 sati
Napomene:
Rijeka within the project “Research Infrastructure for Campus-based Laboratories at University of Rijeka”, financed by European Regional Development Fund (ERDF).



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