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Autori: Broznić, Dalibor; Marinić, Jelena; Tota, Marin; Čanadi Jurešić, Gordana; Milin, Čedomila
Naslov: Protective effects of polyphenols extracted from olive oil on toxicity of imidacloprid
Izvornik: Book of Abstracts of the HDBMB 2006 / Kovarik, Zrinka (ur.). - Zagreb : Hrvatsko društvo za biokemiju i molekulernu biologiju , 2006. 92-92.
Skup: Kongres hrvatskog društva za biokemiju i molekularnu biologiju prigodom 30. obljetnice osnutka uz međunarodno sudjelovanje
Mjesto i datum: Vodice, Hrvatska, 03.-07.10.2006
Ključne riječi: polyphenols; imidacloprid; olive oil
Sažetak:
Imidacloprid [1-(6-chloro-3pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine ; IM] is a new and potent nitromethylene insecticide with high insecticidal activity at very low application rates. As a highly effective insecticide it is used against olive flies in the olive orchards, but owing to its effects on central nervous system and blockade of postsynaptic nicotinergic type of acetylcholine receptors it may be toxic also for the mammalian hosts. In this study we investigated, therefore, the degradation rate of IM in the mouse liver and tested the possibility that polyphenols, extracted from olive oil, have the protective effects on these events. For that purpose experimental animals (C57/BL6 mice) were divided in three groups: (1) intact mice, (2) imidacloprid treated mice (5 mg/kg bwt) and (3) mice pretreated with polyphenol extract (10 mg/kg bwt) for seven days before the administration of IM. Mice were sacrificed 15 min, 1, 6, 12, 24 and 48 h after the treatment with imidacloprid. Liver samples were prepared with a liquid- liquid extraction procedure with dichlormetane and the concentration of imidacloprid and of 6-chloronicotinic acid (6-CNA), which is a metabolic product of IM, was analyzed by HPLC method with UV detection. The data revealed that 6-CNA concentration in the liver obtained a maximum 6h after the administration of IM (p<0.02 in comparison with untreated mice). In mice pretreated with polyphenol extract the concentration of 6-CNA and the half-life of imidacloprid were, however, significantly decreased compared to group 2 (p<0.05), while the maximum of 6-CNA concentration was translocated to an earlier period (15 min). In the both groups the degradation rate of IM was proportional to the dose of administered pesticide, pointing to the suitability of used animal model in this study. Moreover, in group treated with polyphenol extract we found that the rate constant (K) was increased compared to group 2 (p<0.05), as determined with integral and differential method. The data emphasize that polyphenol extracts of olive oils might increase the degradation rate of IM and diminish the toxicity of this pesticide.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Nema recenziju
Projekt / tema: 0062002
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti



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