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Pregled bibliografske jedinice broj: 499752

Zbornik radova

Autori: Ljubunčić, Samra; Zoričić, Petra; Rebić, Danica; Gobin, Ivana
Naslov: The replication of Legionella in alveolar epithelial cells
( The replication of Legionella in alveolar epithelial cells )
Izvornik: Liječnički vjesnik
Skup: 10th ZIMS, Zagreb International Medical Summit for students and young doctors
Mjesto i datum: Zagreb, Hrvatska, 11.-14.11.2010.
Ključne riječi: Legionella; alveolar epithelial cells; replication; infection
( Legionella; alveolar epithelial cells; replication; infection )
Sažetak:
Legionella genus comprise more than 50 species.In humans, these bacteria can cause Legionnaires´disease and Pontiac fewer. It has been proven that Legionella can multiply in alveolar macrophages. There are little information about proliferation of this bacteria in alveolar epithelial cells. Therefore, in this study we examined intracelullar growth of L. longbeachae and L. pneumophila in alveolar epithelial cell line. In this experiment L. longbeachae and L. pneumophila strain were used. Alveolar epithelial cell line (A549) cells was infected with bacteria at an MOI of 10. Bacterial replication was followed for three consecutive days after initial infection. Two hours after infection ~102 bacteria was isolated. After 72 hours there was an exponential growth of L. pneumophila (106 cfu/ml) and L. longbeachae (~105 cfu/ml). The intracellular localization of bacteria has been shown by Giemsa stain. The results indicate that both Legionella species have the ability to replicate in alveolar epithelial A549 cells.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Nema recenziju
Projekt / tema: 062-0621273-1275
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti,Kliničke medicinske znanosti
Upisao u CROSBI: sandraa@medri.hr (sandraa@medri.hr), 25. Sij. 2011. u 14:14 sati



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