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Pregled bibliografske jedinice broj: 499759

Zbornik radova

Autori: Turković, Dastina; Malnar, Maja; Rebić, Danica; Trobonjača, Zlatko; Gobin, Ivana
Naslov: Co-cultivation of Legionella and amoeba Dictyostelium discoideum
Izvornik: Liječnički vjesnik
Skup: 10th ZIMS, Zagreb International Medical Summit for students and young doctors
Mjesto i datum: Zagreb, Hrvatska, 11.-14.11.2010.
Ključne riječi: Legionella; amoeba; co-cultivation; Dictyostelium
Sažetak:
Legionella naturally exploits numerous species of protozoa, which provide nutrients, protect the bacteria from adverse conditions and serve as a vehicle for the colonization of new habitats. D. discoideum is a unicellular, free-living amoeba that lives in soil and water and feeds on bacteria. This report describes the conditions of co-cultivation of Legionella and amoeba D. discoideum. Infection of D. discoideum with Legionella requires an incubation temperature of 25°C. Amoeba cells grown axenically were cultured in HL-5 liquid medium. Legionella were harvested after 48 h growth on BCYE plates, resuspended in water or PBS, and used to infect D. discoideum at an MOI of 10:1. Bacteria and amoeba was co-cultivated in two different media: HL5 media and assay media. The number of viable bacteria on each day was determined by measuring CFU. One day after co-cultivation significantly lower number of L. longbeachae was detected (~102) in comparisom to number of L. pneumophila (~104). The number of L. pneumophila increased during 96 hours when ~105 CFU/well was detected in bouth media. L. longbeachae was able to grew better in assay media and 96 h p.i. ~104 CFU/well was detected. In HL5 media the number of L. longbeachae increased only for 0, 5 log. Importantly, HL5 medium alone or assay media does not support L. pneumophila or L. longbeachae growth, indicating that all growth detected is a result of intracellular multiplication. In conclusion, bouth Legionella are able to infect and proliferate within D. discoideum and replication was better in assay media.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Nema recenziju
Projekt / tema: 062-0621273-1275
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
Upisao u CROSBI: sandraa@medri.hr (sandraa@medri.hr), 25. Sij. 2011. u 14:21 sati



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