Hrvatska znanstvena bibliografija (CROSBI)

Pregled bibliografske jedinice broj: 540975

Zbornik radova

Autori: Raljević, Damir; Peršić, Viktor; Ružić, Alen; Miletić, Bojan; Travica Samsa, Dijana; Rakić, Marijana; Pehar Pejčinović, Vesna; Laškarin, Gordana
Naslov: Changes in frequency of peripheral blood lymphocyte subpopulations in patients with myocardial infarction during a period of early medical rehabilitation
Izvornik: Electronic abstract book
Skup: EuroPRevent 2010
Mjesto i datum: Prag, Češka, 07.-10. 05. 2010.
Ključne riječi: lymphocyte subpopulation; myocardial infarction; peripheral blood
Purpose: The site of infarction and remote myocardial regions are characterized with inflammatory reaction and leukocyte infiltration. However, there is insufficient knowledge about the involvement of peripheral blood lymphocyte subpopulations in this inflammatory process. The purpose of the study was to investigate changes in the frequency of lymphocyte subpopulations in peripheral blood of patients with myocardial infarctation (MI) during the early medical rehabilitation period. Methods: Twelve patients with ST elevation MI (STEMI) and diminished possibility for further progression of the coronary artery disease due to percutaneous coronary intervention and 12 patients with non-STEMI who were treated with standard drug therapy were enrolled in this pilot study. The group of twenty healthy volunteers was used as the control. Peripheral blood samples were taken from MI patients on day 7, 14, 21 and 28 after the acute coronary event and peripheral blood mononuclear cells (PBMC) were immediately isolated by gradient density centrifugation. The frequencies of lymphocyte subpopulation were analyzed by simultaneous assessment of surface markers using fluorochrome conjugated monoclonal antibodies directed toward CD3 and CD56 molecules. T cells were distinguished from the other lymphocyte subpopulations as cells labeled with anti-CD3 monoclonal antibody, but negative for CD56 staining (CD3+CD56- cells). The cells stained as CD3-CD56+ were determined as NK cells, whereas CD3+ and CD56+ cells were counted as NK/T cells. Results: In patients with non-STEMI the frequency of T cells decreased on day 7 and 14 and in the patients with STEMI on day 14 and 21 after the acute myocardial ischemia in comparison with healthy persons. At the end of early rehabilitation period on day 28 the percentage of T cells is restored in both groups of patients. The frequency of NKT cells in peripheral blood of patients with non-STEMI increased on day 14 when compared with healthy volunteers and patients with STEMI. On day 21 and 28 the percentage of NKT cells did not differ from the control. The frequency of NK cells did not significantly change at time points investigated. Conclusions: The fluctuation of peripheral blood T and NKT cells during early medical rehabilitation period suggests their participation in myocardial remodeling events. The investigation of their activation status would provide better insight in immune response and it would open possibilities for new approaches in early medical rehabilitation of patients with MI.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 062-0620402-0377
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti,Kliničke medicinske znanosti
Upisao u CROSBI: (, 15. Stu. 2011. u 21:53 sati