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Pregled bibliografske jedinice broj: 986666

Časopis

Autori: Peruč, Dolores; Tićac, Brigita; Abram, Maja; Broznić, Dalibor; Štifter, Sanja; Malenica Staver, Mladenka; Gobin, Ivana
Naslov: Synergistic potential of Juniperus communis and Helichrysum italicum essential oils against nontuberculous mycobacteria
( Synergistic potential of Juniperus communis and Helichrysum italicum essential oils against nontuberculous mycobacteria )
Izvornik: Journal of Medical Microbiology (0022-2615) 68 (2019), 5; 703-710
Vrsta rada: članak
Ključne riječi: antiadhesion ; antibiofilm ; checkerboard synergy method ; essential oils ; nontuberculous mycobacteria, opportunistic premise plumbing pathogens
( antiadhesion ; antibiofilm ; checkerboard synergy method ; essential oils ; nontuberculous mycobacteria, opportunistic premise plumbing pathogens )
Sažetak:
Objective. The present study evaluated the possible synergistic antimycobacterial interactions of Juniperus communis (J. communis) and Helichrysum italicum (H. italicum) essential oils (EO). Methods. Antimycobacterial potential was tested against Mycobacterium avium and Mycobacterium intracellulare using broth and water dilution method and checkerboard synergy method. Antiadhesion and antibiofilm effect of EOs was evaluated on biotic (HeLa cells) and abiotic surface (polystyrene). To evaluate the possible mechanisms of action, cellular leakage of proteins and DNA was tested and structural changes were visualized with a transmission electron microscope. Results. MIC, MBC and MEC were 1.6 mg/ml for J. communis EO and 3.2 mg/ml for H. italicum EO against both mycobacteria. All combinations of EOs in checkerboard synergy method produced fractional inhibitory concentration index values ranging from 0.501 to 1.5, corresponding to synergistic, additive or indifferent effects. Mycobacterium avium showed a greater tendency to create biofilm but these EO at subinhibitory concentrations (sMIC) effectively blocked the adhesion and the establishment of biofilm. The exposure of both mycobacteria to MICs and sMICs lead to significant morphological changes: acquired a swollen form, ghost-like cell, disorganized cytoplasm detached from the cell wall. OD value of supernatant for both mycobacteria exposed to EOs have confirmed that there is a leakage of cellular material. Conclusion. The leakage of the cellular material is noticeably higher in sMIC, which is probably due to cell wall damage. sMIC of both EOs have an additive or synergistic effect, reducing MICs, limiting adhesion and preventing the formation of biofilms.
Projekt / tema: University of Rijeka (grants No. 13.06.2.2.60 and 13.06.1.1.07)
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti,Kliničke medicinske znanosti
URL Internet adrese: https://jmm.microbiologyresearch.org/content/journal/jmm/68/5
Broj citata:
Altmetric:
DOI: 10.1099/jmm.0.000962
URL cjelovitog teksta:
Časopis izlazi u samo elektroničkom izdanju: NE
Google Scholar: Synergistic potential of Juniperus communis and Helichrysum italicum essential oils against nontuberculous mycobacteria
Upisao u CROSBI: Dolores Peruč (dolores.peruc@uniri.hr), 25. Vel. 2019. u 18:56 sati



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